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. 2023 Jan 4;24(3):e55762. doi: 10.15252/embr.202255762

Figure 5. METTL3 protects METTL14 from STUB1‐mediated ubiquitination to maintain m6A homeostasis.

Figure 5

  1. Immunoblots showing METTL14 protein levels under METTL3 knockdown with or without 10 μM MG132 treatment. β‐actin was used as the loading control. The METTL3 or METTL14/β‐actin densitometric ratio was recorded by ImageJ.
  2. METTL14 ubiquitination, as detected by IP of METTL3 truncated mutants with anti‐HA antibody. The accumulation of Ub and METTL14 was confirmed in whole‐cell lysates. β‐actin was used as the loading control.
  3. Immunoblots showing METTL14 protein levels, as detected by IP in response to METTL3 truncated mutants and STUB1‐FLAG. β‐actin was used as the loading control.
  4. Dot blot assays showing the activities of METTL3 truncated mutants and STUB1‐FLAG in regulating m6A levels.

Source data are available online for this figure.