FIG. 1.

Targeted disruption of Sox8 in mice. (A) Schematic representation of the targeting vector (top), the Sox8 wild-type locus (middle), and the mutant locus (bottom). The three known Sox8 exons are shown as boxes; introns and flanking regions are shown as bars. Regions of homology between wild-type locus and targeting vector are in black. Restriction sites for BamHI (B), BglII (Bg), EcoRI (E), MscI (M), and XbaI (X) are shown, as well as the localization of 5′ and 3′ probes. (B) Southern blot analysis of DNAs from adult wild-type (+/+), heterozygous (+/−), and homozygous (−/−) mice digested with EcoRI for use of the 5′ probe. (C) Southern blot analysis of DNAs from mice of all three genotypes digested with BamHI for use of the 3′ probe. The sizes of bands corresponding to the wild-type and the targeted alleles are indicated. (D) Analysis of Sox8 expression in various tissues of wild-type (+/+) and homozygous (−/−) adult mice by RT-PCR using primer pairs specific for the coding regions of the Sox8, lacZ, and GAPDH genes.