FIG. 2.
Developmental expression of Sox8 and Sox8lacZ. β-Galactosidase activity was detected colorimetrically using X-Gal substrate in age-matched whole-mount (A to C) and vibratome-sectioned (E to I) heterozygous (A to F) and homozygous (G to I) embryos at 9.5 dpc (A), 10.5 dpc (B), 11.5 dpc (C), 12.5 dpc (G), 14.5 dpc (E and H), and 16.5 dpc (F and I). Age-matched heterozygous and homozygous embryos were stained in parallel for identical times. No lacZ staining was detected in wild-type littermates under the conditions used. In panel A, the magnified area corresponds to the boxed region of the embryo. Panel F is a composite of two adjacent sections. Panel D shows in situ hybridization of a vibratome-sectioned embryo at 12.5 dpc with a Sox8-specific antisense riboprobe. The ventral surface is always to the left. Abbreviations: ba, branchial arches; c, cochlea; cg, cranial ganglia; d, diencephalon; drg, dorsal root ganglion; e, eye; fp, facial process; ge, ganglionic eminence; h, heart; i, intestine; k, kidney; l, limb bud; np, nasal pit; mo, medulla oblongata; m, mesencephalon; ob, olfactory bulb; oc, optic cup; op, otic placode; p, pons; so, somite; sc, spinal cord; s, striatum; t, telencephalon; to, tongue.