Skip to main content
. Author manuscript; available in PMC: 2023 Mar 6.
Published in final edited form as: J Am Chem Soc. 2020 Sep 4;142(37):15697–15710. doi: 10.1021/jacs.0c02088

Figure 2:

Figure 2:

(A-B) smFRET efficiency (E) histograms of Sic1 (A) and pSic1 (B) labeled with Alexa Fluor 488 and Alexa Fluor 647 at positions −1C and T90C in TE buffer pH 7.5 150 mM NaCl. (C) Example SAW homopolymer P(ree) distributions (left vertical scale) for Sic1 (black) and pSic1 (red). The shaded underlying region shows the FRET distance dependence function E(ree) (right vertical scale). (D) Dimensionless Kratky plots of Sic1 (black) and pSic1 (red), normalized by initial intensity I0 and the Rg estimated from the DATGNOM[49] fit of the distance distribution function. (E) Guinier plots of Sic1 (black) and pSic1 (red). The solid circles are the data points selected for fitting a restricted range appropriate for IDPs (qmaxRg < 0.9) and the solid lines show the Guinier fits using these data points. (F) The normalized distance distribution function P (r) estimated by DATGNOM for Sic1 (black) and pSic1 (red).