Fig. 2.

Loss of STAT1 in the cancer cell has an anti-tumoral and anti-proliferative effect a Experimental design for murine orthotopic tumor models treated with hypofractioned RT. LY2 and MOC2 tumors were established in BALB/c and C57BL/6 mice, respectively. Administration of RT (24 Gy in 3 fractions) over approximately 1 week began when tumors from respective groups were approximately 150–200 mm³, given on days 8, 12, and 15 for MOC2 PX458, days 15, 18, and 21 for MOC2 STAT1 KO, days 24, 28, and 32 for LY2 PX458, and days 32, 36, and 40 for LY2 STAT1 KO. b Tumor growth curves for LY2 STAT1 KO versus LY2 PX458 Ctrl with or without RT; replicate values shown as bar chart for all mice alive on day indicated by vertical line; unpaired two-tailed t test. c Tumor growth curves for MOC2 STAT1 KO versus MOC2 PX458 Ctrl with or without RT; replicate values shown as bar chart for all mice alive on day indicated by vertical line; unpaired two-tailed t test. d Percent confluence for indicated cancer cell lines; unpaired two-tailed t test calculated at final time point