Immunofluorescence staining for fibulin-1 and fibulin-2 of heart valve (A to D) and myocardium including a large artery (E to H). Staining was performed with equal concentrations of antibodies against fibulin-1 (A, B, E, and F) and fibulin-2 (C, D, G, and H). Heterozygous and homozygous embryos were from stage E18.5. Bar, 100 μm.