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. 2023 Mar 6;18(3):e0282569. doi: 10.1371/journal.pone.0282569

Fig 5. Metabolic influence of TRPA1 is concentrated in tyrosine metabolism.

Fig 5

(A-C) Individual pathways as indicated from Metaboanalyst taken from metabolites identified as different between wild type and TRPA1-/- mice in response to TDI (A), R. mucosa treated versus R. mucosa plus Cardamonin (RmC; B), and R. mucosa treated versus R. mucosa plus ground cardamom seeds (RmS; C). (D) Summarizing all pathways impacted by index of pathway significance (IPS) for each condition versus its diluent control. (E, F) Percent of starting wound closure over time for keratinocytes (E) and Schwann neuron cells (F) incubated with diluent, the TRAP1 agonist cinnamaldehyde (Cinn) or TRPA1 blocker HC030031 (N = 1 cells line each in triplicate wells). Significance determined by comparison of area under the curve with 95% confidence intervals by PRISM. (G) Schwann cells in scratch assay with Cinn with or without addition of anti-dopaminergic haloperidol (halo) or anti-adrenergic sotalol (N = 1 cells line each in triplicate wells). Data represent two or more independent experiments and are shown as mean ± SEM. * = p < 0.05 for comparison of area under the curve for wound closure as indicated.