FIG. 2.

The addition of NF-Y subunits does not reverse CIITA-mediated suppression of the collagen α₂(I) promoter. (A) The four panels show that the addition of exogenous NF-YA, NF-YB, NF-YC, or a combination of the three had little to modest effects on CIITA-mediated suppression of the collagen promoter. The NF-Y subunits, alone or in combination, were examined for their capacity to reverse CIITA-mediated suppression of the collagen promoter. All the experiments were repeated three times. (B) The expression of NF-YA, NF-YB, and NF-YC in COS7 cell transfectants was confirmed by immunoblotting (IB) using anti-Flag M5 antibody.