Figure 1.

(a) Mouse immunization campaign pipeline. (b) A workflow for high-throughput discovery of cross-reactive anti-uPAR antibodies using the bacon platform. (c) Each ASC was cultured in individual nanopens to allow secreted antibodies to be accumulated. After culturing cells for 1–2 hours, anti-mouse IgG (H+L)-coated beads are imported into the channel along with fluorescently labeled uPAR (AF488-human suPAR, green; and AF647-cyno uPAR, red). As the secreted antibodies are immobilized on the beads, antigen recognition allows for a time-dependent accumulation of fluorescent signals immediately above each nanopen. (d) The increased signal in AF488 and AF647 channels was observed between T0 and T11, suggesting the antibodies were able to recognize human/cyno uPAR.