Figure 6.

(a) Lead antibodies were internalized by MDA-MB-231 cells and colocalized with lysosomes. Scale bars = 10 µm. (b) Fluorescent signal overlap between antibodies and lysosomes is represented quantitatively using the manders’ coefficient. A high degree of overlap is observed for all lead antibodies, suggesting active lysosomal uptake. (c) Quantification of relative uPAR expression in MDA-MB-231 and uPAR-knockdown MDA-MB-231 cells from the immunoblot result. (d) On-target binding of uPAR on MDA-MB-231 and uPAR-knockdown MDA-MB-231 cells by flow cytometry.