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. 2023 Feb 1;324(3):F315–F328. doi: 10.1152/ajprenal.00079.2022

Table 2.

Primers used in the present study

Protein	Primer
Protein	Forward (5′–3′)	Reverse (3′–5′)	Product Size, bp
Cullin-1	`CACCTCCACACCAGAGGTTT`	`TGGAGACGCACATCTGAAAG`	174
Cullin-2	`TTTTCCTGGCAGCTAGCATT`	`ATCGGAACTGACACCACACA`	154
Cullin-3	`GTGTTTCCCTTGCTCCACAT`	`ACGGCAACAAGCCAAATAAC`	227
Cullin-4A	`TTGCTGCTTTGTCCCTCTTT`	`GTGTGAAAGCCAAGCTCCTC`	180
Cullin-4B	`CTGAGCTCTAATGCCCAAGG`	`TCTCTGGAACAGGAGGCACT`	218
Cullin-5	`GTGAGCCTTTGAGCATGTGA`	`CACAGGTGGGACTCCACTTT`	233
Cullin-7	`TCATTGTCTGAGCGTCCTTG`	`TTACAGATGGTTGCGAGCTG`	194
Cullin-9	`CAGATGAGGGGATGGAGAAA`	`TCACACCACTGTGACGGATT`	165
GAPDH	`AGACAGCCGCATCTTCTTGT`	`CTTGCCGTGGGTAGAGTCAT`	207