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Preparation of osmotic pressure-adjusted culture medium
| Culture Medium | Mosmol/kg | dDW, mL | DMEM, mL | 1.4% NaCl in dDW, mL |
|---|---|---|---|---|
| Isotonic medium | 330 | 3.5 | 4 | 0.5 |
| Middle tonic medium | 300 | 3.0 | 4 | 1.0 |
| Hypotonic medium | 270 | 2.5 | 4 | 1.5 |
| (reference) | ||||
| DMEM 10% FBS | 336 | |||
| DMEM FBS free | 336 |
Calcium concentration of all medium is 1.8 mM.
