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CD3 cross-linking enhances NF-AT transcriptional activity in Jurkat cells but not P116 cells. Parental Jurkat cells and P116 cells were transfected with the luciferase reporter construct driven by NF-AT. These cells were subsequently stimulated with the indicated dosages of UCHT1 or 50 ng of PMA per ml (defined as the maximal response) for 6 h before being analyzed for luciferase activity. The results shown are expressed as the percentage of the maximal response to PMA.
