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. 2023 Mar 6;14(3):183. doi: 10.1038/s41419-023-05701-7

Fig. 1. TRPM7 knockout impairs glucose catabolism.

Fig. 1

A Gene set enrichment analysis (GSEA) of Hallmark gene sets in TRPM7KO versus WT T24 cells. B Glucose uptake analysis by 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose (2NBDG). C–E Glycolysis was measured by Extracellular acidification rates (ECAR) in WT and TRPM7KO cells under basal conditions and in response to 10 mM glucose, 3 μM oligomycin or 100 mM 2-deoxy-D-glucose (2-DG). F Relative intracellular lactate in WT or TRPM7KO cells. G Glycolytic intermediates and metabolites quantified by mass spectrometry. Two groups of samples were compared by unpaired two-tailed Student’s t-test.