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. 2023 Mar 6;14(3):183. doi: 10.1038/s41419-023-05701-7

Fig. 5. The CRTC2/CREB axis acts downstream of TRPM7/Ca2+/calcineurin to regulate SLC2A3 transcription.

Fig. 5

A GSEA of the CREB DNA-binding element gene sets in WT or TRPM7KO cells. B, C Immunoblot analysis of CREB signaling in WT and TRPM7KO cells. D Immunoblot analysis of CREB signaling in response to naltriben (25 μM), FK506 (preincubation, 1 μM) or forskolin (10 μM). E, F Nuclear translocation of CRTC2 in response to naltriben (25 μM, 2 h), FK506 (preincubation, 1 μM, 1 h) or forskolin (10 μM, 2 h) (scale bars, 20 μm). Two groups of samples were compared by unpaired two-tailed Student’s t-test. Multiple groups were compared by ANOVA.