Skip to main content
. 2023 Feb 21;13:1058337. doi: 10.3389/fonc.2023.1058337

Figure 4.

Figure 4

S100A4, SPARC and SPP1 are expressed by tumor-associated macrophages in colorectal cancer. (A) IHC representative images (x400) of stromal S100A4, SPARC and SPP1 in tumor tissue. Zoom images is given in the lower panel (x800). Stromal expression of S100A4 and SPARC is higher than tumor expression. The protein level of SPP1 does not have differences between tumor and stroma. Student’s t-test was applied. (B) Immune cell-type deconvolution analysis that was performed via the TIMER2.0 web platform using the CIBERSORT method. S100A4, SPP1 and SPARC gene expression correlates with macrophages. *: |rho|>0.2 and FDR<0.05. (C) Nanostring GeoMX DSP revealed increased expression of SPP1 in the immune compartment and its correlation with macrophages. *: |rho|>0.2 and FDR<0.05. (D) Confocal microscopy confirmed the co-localization of S100A4, SPARC and SPP1 in CD68+ TAMs. Scale bars equal 20 µm.