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mekk1-null DT40 cells remain arrested at G2/M after prolonged vinblastine treatment, but wild-type DT40 cells undergo apoptosis after 8 h of vinblastine treatment. DT40 or MC2 cells (106) were treated for 6 or 8 h with 1 μM vinblastine sulfate or for 8 h with DT40 medium alone. Cells were stained in hypotonic DNA staining buffer and analyzed as described in Materials and Methods.
