Table 2.
PCR primers used to amplify ACCase cDNA of D. ciliaris var. chrysoblephara.
| Primer | Sequence (5’to 3’) | Annealing temperature (°C) | Gene location |
|---|---|---|---|
| 5GSP | TCTCCTCAGGTATCGAGTCC | For nested PCR | For nested PCR |
| 5GNP | AGGCCATCCAAGAATCAAAG | ||
| A.C-F2 | TTCAGCTCTCATTGCTCAAG | 59.3 | 777–1,971 |
| A.C-R2 | TAGAGCTCCTCCAACCACTG | ||
| A.C-F3 | ATTCAAATTCGTGGAGAAAT | 55.8 | 1,801–3,153 |
| A.C-R3 | AGCTTTGTTCCTCACACCCT | ||
| A.C-F4 | TTTTCAGTGATGGCATTCAG | 57.6 | 3,038–4,052 |
| A.C-R4 | CGTGATGGAGTATACTTCAT | ||
| A.C-F5 | AGATCAGATTCTCCGGCATG | 56.4 | 3,951–5,031 |
| A.C-R5 | TGGGCCAAATGATCCAGCTC | ||
| A.C-F6 | TTACTAGTCACACCTGTACAG | 57.6 | 4,544–5,683 |
| A.C-R6 | TAGGAAGAGGTCCACCAATG | ||
| A.C-F7 | GACTGTTTCAGATGACCTTG | 55.9 | 5,598–6,631 |
| A.C-R7 | AAGGATATCTGAAGATGTTC | ||
| A.C-F8 | TGTTATGCTGAGAGGACTG | 56.0 | 6,259–6,966 |
| A.C-R8 | CAGCCGCCTTGTATCCATCT |