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. 2001 Nov;21(21):7207–7217. doi: 10.1128/MCB.21.21.7207-7217.2001

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Copyright © 2001, American Society for Microbiology

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FIG. 3 — Coimmunoprecipitation of NIK, TAK1, IKKα, and IKKβ with RKIP. (A) FLAG-RKIP was cotransfected into COS-1 cells with the indicated HA-tagged expression plasmids. Extracts were immunoprecipitated (IP) with anti-HA or anti-FLAG antibodies as indicated. Immunoprecipitates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to membranes, and the expression levels of HA-tagged or FLAG-tagged proteins were monitored by immunoblotting (IB) with the indicated antibodies. (B) HA-RKIP was cotransfected into COS-1 cells with the indicated FLAG-tagged expression plasmids. Extracts were analyzed as indicated above. (C) HA-RKIP was cotransfected into COS-1 cells either alone or with FLAG-tagged IKKα or IKKβ expression plasmids. Extracts were analyzed as indicated above.