Table 1.
Specification of monoclonal antibodies (mAb)
| Antigen | CD | Ab clone | Source | Isotype | Conjugate | Provider |
|---|---|---|---|---|---|---|
| Isotype control | n.c. | MOPC-21 | mouse | IgG1 | PE | BD Biosciences |
| Isotype control | n.c. | MOPC-21 | mouse | IgG1 | Alexa Fluor 647 | BD Biosciences |
| Isotype control | n.c. | 20102 | mouse | IgG2a | PE | R&D Systems |
| Isotype control | n.c. | 133303 | mouse | IgG2b | PE | R&D Systems |
| HPCA-1 | CD34 | 581 | mouse | IgG1 | PE | BD Biosciences |
| T10 | CD38 | HIT2 | mouse | IgG1 | APC | BD Biosciences |
| LCA | CD45 | HI30 | mouse | IgG1 | APC-Cy7 | Biolegend |
| LAMP-3 | CD63 | CLB-gran12 | mouse | IgG1 | PE | Immunotech |
| Transferrin-R | CD71 | RI7217 | mouse | IgG2a | PE | Biolegend |
| APO-1, FAS | CD95 | DX2 | mouse | IgG1 | PE | BD Biosciences |
| SCF-R/KIT | CD117 | 104D2 | mouse | IgG1 | PE | BD Biosciences |
| SCF-R/KIT | CD117 | 104D2 | mouse | IgG1 | PE-Cy7 | Invitrogen |
| EMR2 | CD312 | REA302 | mouse | IgG1 | PE | Miltenyi Biotec |
| MRGX2 | n.c. | K125H4 | mouse | IgG2b | PE | Biolegend |
| pAKT | n.c. | M89-61 | mouse | IgG1 | PE | BD Biosciences |
| pS6 | n.c. | N7-548 | mouse | IgG1 | Alexa Fluor 647 | BD Biosciences |
| pSTAT5 | n.c. | 47 | mouse | IgG1 | Alexa Fluor 647 | BD Biosciences |
Abbreviations: CD, Cluster of Differentiation; Ab, Antibody; n.c., not yet clustered; IgG, Immunoglobulin G; PE, Phycoerythrin; HPCA-1, Human Precursor Cell Antigen-1; APC, Allophycocyanin; LCA, Leukocyte Common Antigen; LAMP-3, Lysosome-Associated Membrane Glycoprotein 3; R, Receptor; Cy7, Cyanine7; APO-1, Apoptosis Antigen 1; SCF-R, Stem Cell Factor Receptor; EMR2, EGF-Like Module-Containing Mucin-Like Hormone Receptor-Like 2 (Implicated In Vibration-Induced Urticaria); MRGX2, Mas-Related G-Protein Coupled Receptor Member X2; pAKT, Phosphorylated AKT Kinase; pS6, Phosphorylated Ribosomal Protein S6; pSTAT5, Phosphorylated Signal Transducer and Activator Of Transcription 5; Location of providers: BD Biosciences, San José, CA, USA; R&D Systems, Minneapolis, MN, USA; Immunotech, Marseille, France; BioLegend, San Diego, CA, USA; Invitrogen, Carlsbad, CA, US; Miltenyi Biotec, Bergisch Gladbach, Germany.