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. 2023 Jan 31;42(2):112059. doi: 10.1016/j.celrep.2023.112059

Figure 1.

Figure 1

NPY levels correlate with liver disease in mice and in human hepatic cirrhosis

(A) Serum NPY ELISA from control and cirrhotic patients undergoing transjugular intrahepatic portosystemic shunt (TIPS) treatment, n = 7 (TIPS-group, cirrhotic patients) and n = 6 (controls), ∗∗p < 0.02 for cirrhotic patients vs. healthy individuals (absolute values).

(B) Serum NPY ELISA in cirrhotic patients shows increased NPY in Child-Pugh class C compared with Child-Pugh class A patients, n = 5 per group, ∗p < 0.05 for Child-Pugh class C vs. Child-Pugh class A cirrhotic patients. Results are expressed as mean ± standard error of the mean (SEM).

(C) Serum NPY ELISA from portal vein (PV, hepatic inflow) and hepatic vein (HV, hepatic outflow) in TIPS patients, n = 16 per group, ∗∗p < 0.02 for PV vs. HV.

(D) Serum NPY ELISA from hepatic vein (HV) and hepatic artery (HA) in n = 21 per group, n.s. (not significant).

(E) NPY levels compared with portal vein and hepatic artery, ∗∗∗p < 0.001.

(F) In silico analysis of hepatic mRNA NPY levels compared with TGFB1 expression in human liver tissues.

(G) Hepatic Nep and Col1A1 mRNA expression in patients with chronic liver disease; number of XY pairs = 17, r = 0.7472, p = 0.0006.

(H and I) Hepatic Nep mRNA expression from bile duct ligation (BDL)-treated and CCl4-treated WT (C57BL/6) mice for 2 and 4 weeks, respectively, ∗∗p < 0.02 for BDL- or CCl4-treated vs. corresponding control mice. Data were normalized to the expression of 18S RNA.

(J) Liver homogenates NPY ELISA from WT and Nep−/− mice, control vs. CCl4, ∗p < 0.05.

(K) Morphometric analysis of Sirius red staining of livers from WT mice compared with Nep−/− with and without NPYFL (left panel) and WT BDL mice vs. Nep−/− BDL with and without NPYFL (right panel). n = 5 per group, ∗∗p < 0.01, ∗∗∗p < 0.001, and n.s. (non-significant).

(L) Liver sections stain with Sirius red. Scale bar: 200 μm.

(M) Col1A1 mRNA expression of WT mice comparing non-treated controls vs. animals that were injected with full-length NPY, ∗∗p < 0.01. Col1A1 mRNA expression of Nep−/− mice after profibrotic stimuli (BDL) comparing control vs. Nep−/− after injecting full-length NPY, ∗∗p < 0.01. Data were normalized to the expression of 18S RNA.