FIG. 1.

Targeted deletion of the Tnp2 gene. (A) Genomic Tnp2 locus, the replacement vector used, and the targeted locus. Restriction sites: A, AvaI; B, BamHI; Bs, BsmI; C, ClaI; E, EcoRI; H, HindIII; N, NotI. The positions of the probes used to distinguish the targeted locus from the endogenous locus are indicated. (B and C) Southern blot genotyping performed by probing BamHI digests with the 5′ probe (B) or probing EcoRI digests with the 3′ probe (C). (D) PCR genotyping using primers for Tnp2 and neo.