Fig. 1.

(A) Chemical structures of curcumin and its analog GO‐Y030. (B, C) Effects of GO‐Y030 on CSC sphere‐forming ability of the cancer cell lines. PC3 and HuH7 cells were plated as single cells on ultra‐low attachment 24‐well plates at a density of 200 cells per well in serum‐free medium at 37 °C for 7 and 12 days, respectively. Statistical significance was calculated using Student's t‐test. Data represent the mean ± SEM of three independent experiments. *P < 0.05 and **P < 0.01 compared to the control group (dimethylsulfoxide alone). Scale bar = 100 μm.