Fig. 3.

Inhibitory effect of GO‐Y030 on refolding activity of intracellular chaperone. PC3 (upper) and 293T (lower) cells were transiently transfected with an expression construct coding luciferase protein. Transfected cells were treated with GO‐Y030 or PFT‐μ at the indicated concentrations, subjected to heat shock (43 °C, 30 min), and then cultured at 37 °C for 6 h before the luciferase assay. The refolding activity of the control group treated with dimethylsulfoxide alone was set as 100%. Statistical significance calculated using Student's t‐test. Data represent the mean ± SEM of three independent experiments. **P < 0.01 compared to the control group (dimethylsulfoxide alone).