FIG. 10.

Endogenous IB1 gene expression is relieved in TSA-treated cells. Total RNAs from HeLa, NIH 3T3, Jurkat, and RAW cells treated in the presence of DMSO (−) or 100 nM TSA (+) were analyzed for IB1 gene expression by RT-PCR using specific primer pairs as described in Materials and Methods. RNA from βTC3 cells was used as a positive control for IB1 gene expression. In REST-expressing cells, the PCR yielded one PCR fragment detected only in the presence of TSA-treated cells.