FIG. 5.

Mutation of the NRSE motif in the human IB1 promoter relieved transcriptional activity in REST-expressing cell lines. The IB1 promoter containing mutated or wild-type NRSE, mIB1LUC and IB1LUC, respectively, was transiently transfected into REST-expressing HeLa, NIH 3T3, Jurkat, and RAW cells. The luciferase activity was relieved in all these cells with mIB1LUC. Each experiment was performed at least three times in triplicate. Luciferase activities were normalized using pRLCMVrenilla, and results are expressed as means ± standard errors of the means (triple asterisk, P < 0.001). RLU, relative light units.