FIG. 6.

REST represses IB1 promoter activity. IB1LUC and mIB1LUC constructs were cotransfected in the insulin-secreting cell line (βTC3), with REST expression vector RIP REST (under the control of the RIP) or the empty RIP vector as a control. The luciferase activity of IB1LUC was decreased by 50% in REST-expressing cells, whereas REST expression had no effect on mIB1LUC activity. Each experiment was performed at least three times in triplicate. Luciferase activities were normalized using pRLCMVrenilla, and results are expressed as means ± standard errors of the means (asterisk, P < 0.05). RLU, relative light units.