Expression of DNREST derepressed the IB1 promoter and heterologous SV40 promoter activities in HeLa cells. (A) IB1LUC and mIB1LUC vectors were transiently transfected with or without DNREST into HeLa, NIH 3T3, Jurkat, and RAW cells. The IB1LUC activity was relieved with the expression of DNREST. Triple asterisk, P < 0.001. RLU, relative light units. (B) The heterologous construction WTNRSE was transfected in HeLa, NIH 3T3, Jurkat, and RAW cells with or without DNREST. The WTNRSE activity was dramatically decreased compared to the SV40LUC basal activity. The WTNRSE activity was completely restored upon DNREST expression. Triple asterisk, P < 0.001.