FIG. 9.

IB1 promoter activity is repressed through NRSE in a TSA-sensitive manner. (A) IB1 promoter construct IB1LUC was transiently transfected into HeLa, NIH 3T3, Jurkat, and RAW cells. TSA (100 nM) or DMSO as a control was added to medium 24 h after transfection. The IB1LUC activity was relieved in all TSA-treated cells. (B) The mIB1LUC construct was also transfected in the REST-expressing cells with TSA or DMSO. No significant differences in mIB1Luc activity between TSA-treated cells and DMSO-treated cells were observed. (C) The heterologous WTNRSE construct was also transiently transfected in the presence of TSA or DMSO. The WTNRSE activity was restored in all TSA-treated cells. Each experiment was performed at least three times in triplicate. Triple asterisk, P < 0.001. RLU, relative light units.