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. Author manuscript; available in PMC: 2023 Mar 7.
Published in final edited form as: Liver Int. 2020 Feb 6;40(4):830–843. doi: 10.1111/liv.14370

Fig. 5. Inhibition of GPR-91 receptor expression by an RNAi approach abrogated succinate-stimulated HSC activation.

Fig. 5

A&B. Total protein levels of the GPR-91 receptor in immortalized rat BSCC-10 and human HSC cells transduced with a lentivirus harboring scrambled shRNA (Control) or shRNA against the GPR-91 receptor (H-GPR91-KD, R-GPR91-KD) were analyzed by Western blot analysis. C&D. mRNA levels of α-SMA, Col-Iα1, CTGF and GPR-91 in immortalized rat BSCC-10 and human HSC cells transduced with a lentivirus harboring a scrambled shRNA (Control) or shRNA against GPR-91 (H-GPR91-KD, R-GPR91-KD). E&F. Total protein levels of α-SMA, GPR-91, p-Erk1/2, p-c-Jun in immortalized rat BSCC-10 and human HSC cells transduced with a lentivirus harboring a scrambled shRNA (Control) or shRNA against GPR-91 (H-GPR91-KD, R-GPR91-KD). Scram = Scrambled, KD = knockdown with shRNA. The experiment was repeated for at least 3 times. All data were expressed as mean ± SEM. ** p < 0.01 compared to Scram-BSA. △, △△ p < 0.05 and 0.01 compared to Scram-SUC. The ANOVA variance test was used to compare between groups, and the LSD test was used for multiple comparisons between two given groups.