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. Author manuscript; available in PMC: 2023 Mar 7.
Published in final edited form as: Sci Transl Med. 2021 Jul 28;13(604):eaax8891. doi: 10.1126/scitranslmed.aax8891

Fig. 7. Farnesol fails to rescue α-syn PFF–mediated DA neuron loss in Zfp746(PARIS)C638S/C638S knock-in mice.

Fig. 7.

(A) Phosphate-buffered saline (PBS) or α-syn PFF was injected into the STR of 2-month-old PARIS C638S KI (C638S K) mice, and age-matched littermate controls (WT) were fed with control chow or farnesol diet, 2 weeks after injection. (B) Representative immunoblot image of farnesylated PARIS, PGC-1α, PARIS, and FTase α in the SN of C638S KI and WT mice ± α-syn PFF ± farnesol diet at 6 months after injection of α-syn PFF; n = 3 mice per group. Quantitation of the immunoblots in the bottom normalized to immunoprecipitated PARIS or β-actin. WB, western blot analysis. (C) TH staining of a representative section of 8-month-old C638S KI and WT mice ± α-syn PFF ± farnesol diet. Scale bar, 400 µm. Stereological TH, Nissl-positive neuronal counting was indicated at the bottom, n = 4 mice per group. (D) HPLC assessment of the content of dopamine (DA) normalized to protein expression in the STR of 8-month-old C638S KI and WT mice ± α-syn PFF ± farnesol diet; n = 4 mice per group. (E) Assessment of DA-related motor performance by pole test for C638S KI and WT mice ± α-syn PFF ± farnesol diet. WT, n = 8, 9, 10, and 9 mice for each group; C638S KI, n = 8, 10, 9, and 9 mice for each group. Data = means ± SEM. Statistical significance was determined by applying a two-way ANOVA test with Tukey post hoc analysis (C to F). Differences were considered significant when P < 0.05. *P < 0.05, **P < 0.01, and ***P < 0.001. Exact P values can be found in the accompanying statistical raw data.