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(A) The telomere consensus sequence does not correspond to perfect repeats of the sequence specified by the TLC1 template RNA. (B) TLC1 template mutants used in this study. Mutant nucleotides are highlighted. In order to facilitate the cloning, the NcoI restriction site 3′ of the template was destroyed in the mutants by ligation with compatible BspHI ends. This results in a single 455A→G nucleotide change.
