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. 2001 Nov;21(21):7277–7286. doi: 10.1128/MCB.21.21.7277-7286.2001

FIG. 4.

FIG. 4

Telomerase assays using WT and mutant telomerases in vitro. Reaction mixtures contained equal amounts of TLC1 RNA. 5′-GTGTGTGTGGG-3′ was used as a substrate. (A) Lane 1, substrate extended with [α-32P]ddATP using terminal deoxynucleotidyltransferase; lane 2, telomerase reaction from WT cells pretreated with RNase A; and lanes 3 to 11, reactions performed with mutant telomerases as indicated in panel B. The open arrowhead indicates the position of a labeled 32-mer oligonucleotide that served as control for precipitation efficiency and gel loading. The filled arrowhead indicates extension to position 470C. (B) Relative amounts of +1 to +4 products. The different numbers of incorporated radioactive nucleotides were taken into account.