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. 2023 Mar 6;21:78. doi: 10.1186/s12951-023-01835-0

Fig. 1.

Fig. 1

Purification and characterization of ADNVs. A ADNVs were isolated and purified by differential the centrifugation and sucrose gradient ultracentrifugation. B ADNVs harvested from the sucrose density gradient (30%) were characterized by TEM (Scale bar = 200/50 nm). C Particle size of the ADNVs was measured by NanoSight NS 300 system. D Surface charge was measured by a Zetasizer. E ADNVs-enclosed DNA was electrophoresis on a 1.2% agarose gel, and stained with EtBr. F Proteins of ADNVs were separated by 10% SDS-PAGE and stained with Coomassie blue. Shown are the representative results from at least three independent experiments