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. 2023 Mar 7;21:79. doi: 10.1186/s12951-023-01834-1

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Effects of MEP1B on pTr2 cells in vitro. A–E MEP1B promotes pTr2 cell proliferation and migration. A The transfection efficiency of MEP1B overexpression was determined by PCR. B The cell viability of pTr2 cells was applied by CCK-8 assay. C EDU staining assay was performed to determine the cell proliferation changes after MEP1B overexpression. Scale bars = 100 µm. D Wound healing assay for the evaluation of migration of pTr2 cells. Scale bars = 500 µm. E Transwell migration assay revealed that MEP1B overexpression increased the cell numbers of migration. Scale bars = 200 µm. F–J Knockdown of MEP1B inhibits pTr2 cell proliferation and migration. F The transfection efficiency of MEP1B siRNA was determined by PCR. G The cell viability of pTr2 cells was applied by CCK-8 assay. H EDU staining assay was performed to determine cell proliferation changes after MEP1B knockdown. Scale bars = 100 µm. I Wound healing assay for the evaluation of migration of pTr2 cells. Scale bars = 500 µm. J Transwell migration assay indicated that knockdown MEP1B reduced the cell numbers of migration. Scale bars = 200 µm. CCK-8 cell counting kit-8. The data were presented as mean ± SD. *p < 0.05, **p < 0.01, and Student’s t-test