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. 2023 Mar 6;6(5):e202301944. doi: 10.26508/lsa.202301944

Figure S3. TgKI(krt92-p2A-EGFP-t2A-CreERT2);Tg(ubi:CSHm) zebrafish larvae without 4-OHT treatment.

Figure S3.

(A, B, C) Representative confocal images of live zebrafish larvae at 2 dpf (A, A’, A’’), 3 dpf (B, B’, B’’), and 6 dpf (C, C’, C’’). (A’’, B’’, C’’) are merged channels with EGFP and mCherry. The magenta signal is background appearing in pigmented cells and yolk during live imaging. There is no overlap of H2BmCherry with EGFP in the skin, indicating no leakage from residual recombination. (D) Fluorescence microscopic image of live zebrafish embryos at 1 dpf showing the appearance of green fluorescence in the skin. The yellow arrows point to fluorescence-positive embryos. (A, B, C, D) Scale bars = 200 μm (A, B, C) and 500 μm (D).