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. 2023 Mar 6;6(5):e202301944. doi: 10.26508/lsa.202301944

Figure S9. TgKI(krt4-p2A-EGFP-t2A-CreERT2);Tg(ubi:CSHm) zebrafish larvae without 4-OHT treatment.

Figure S9.

(A, B, C) Representative confocal images of live zebrafish larvae at 1 dpf (A, A’, A’’), 2 dpf (B, B’, B’’) and 3 dpf (C, C’, C’’). (A’’, B’’, C’’) are merged channels with EGFP and mCherry. The magenta signal is nuclear (H2BmCherry) and overlapping with EGFP in the skin, indicating leakage from residual recombination in a tissue with very high krt4 expression. The magenta signal in pigmented cells and yolk is background appearing during live imaging. (D) Fluorescence microscopic image of live zebrafish embryos at 2 dpf showing the appearance of green fluorescence in the skin. The yellow arrows point to fluorescence-positive embryos. (A, B, C, D) Scale bars = 200 μm (A, B, C) and 500 μm (D).