FIG. 5.
Two-dimensional gel electrophoresis of hnRNP A1-associated RNP complexes. (A) Proteins in complexes immunopurified with 4B10 from each subcellular fraction of [35S]methionine-labeled cells were resolved by two-dimensional gel electrophoresis as described in the text and visualized by autoradiography. The positions of proteins discussed in the text are indicated. (B) Immunoblot analysis. Nucleoplasmic and Triton-soluble nuclear RNPs resolved by two-dimensional gel electrophoresis as for panel A were immunoblotted with the indicated monoclonal antibodies. The positions of the hnRNP D, E, A1, and C1/C2 proteins and corresponding alternatively spliced isoforms are indicated. Ig h.c., position of the heavy chains from antibodies used for immunopurification.