FIG. 2.

Characterization of growth phenotypes. (A) Growth phenotypes on rich medium. Cultures were grown in rich medium to an OD₆₀₀ of 1.0, and 2 μl of 10-fold serial dilutions was spotted onto agar plates. Plates were grown for 3 days at the temperatures indicated. (B) Recovery of TAF90 mutants from exposure to the restrictive temperature. Cultures were grown in liquid rich medium to an OD₆₀₀ of 1.0, and then 2 μl of 10-fold serial dilutions was spotted onto solid rich medium. Plates were grown either for 3 days at 23°C or for 12 or 24 h at 37°C and then for 3 days at 23°C. Since the cells were plated onto agar plates at room temperature, the exact time that the cells were exposed to a temperature of 37°C is not known.