FIG. 4.
Transcription analysis of specific messages. Cultures were grown, treated, and processed as described in the legend to Fig. 3. (A) S1 analysis of specific messages. Ten micrograms of total RNA was analyzed by S1 nuclease protection (7). tRNAIle, a Pol III transcript, was used as an RNA control. (B) Northern blotting. Fifteen micrograms of total RNA was fractionated on formaldehyde-containing gels and transferred to a membrane, and the specific transcripts were detected by hybridization with 32P-labeled probes corresponding to the coding regions of CLB2, CLN2, TRX1, and RPS5. The Pol III-transcribed scR1 was used as a loading control.