FIG. 6.

Effects of TAF90 mutations on Gcn4p-mediated transcription. Cells were grown under noninducing conditions in synthetic complete medium at 24°C (lanes N) and at 37°C for 3 h (lanes N, 37). A separate culture was grown in synthetic complete medium without histidine, shifted to 37°C for 1.5 h, and incubated for another 1.5 h in the presence of 30 mM 3-aminotriazole (lanes I, 37). Analysis of HIS3 was performed by S1 nuclease protection. The induction level (+13 I/N) is the ratio of the +13 transcript from cells grown under inducing conditions at 37°C to that from cells grown under noninducing conditions at 37°C. The reduced tRNA signal in the taf90-19 sample is a gel-loading artifact and is not reproducible. WT, wild type.