FIG. 9.

Purification of HAT complexes from TAF90 mutants. Extracts prepared from mutants grown at 37°C for 3 h were subjected to chromatographic analysis as described in Materials and Methods. Aliquots of the Mono Q column fractions were analyzed by Western blotting and HAT assays using nucleosomes as substrates. Elution profiles for the wild type (A) and the taf90-20 (B), taf90-17 (C), and taf90-10 (D) mutants are shown. Note that the blots presented in panel D were deliberately exposed longer than those in panels A to C to detect the small amounts of SAGA subunits eluting in fractions 38 to 42.