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. Author manuscript; available in PMC: 2024 Feb 13.
Published in final edited form as: Behav Brain Res. 2022 Nov 4;438:114202. doi: 10.1016/j.bbr.2022.114202

Figure 1A-B. Experimental timeline.

Figure 1A-B.

(A) Timeline outlining experimental study details. After nine generations of unrelated trait-paired mating, animals were screened on the EPM on PNDs 25 and 49. Following the last EPM screen, the three-week CMS treatment commenced. Estrous cycling sampling for E2 occurred one month after CMS. Three FSTs were conducted: FST1 prior to the onset of CMS, FST2 following the three-week CMS regimen, and a terminal FST (data not recorded) prior to animal sacrifice. (B) CMS protocol. Schematic depicting seven mild stressors that comprised the CMS procedure.