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. 2023 Feb 22;8(4):e165713. doi: 10.1172/jci.insight.165713

Figure 6. IGF-1 downregulates markers of systemic oxidative stress and decreases C-reactive protein and chemokine CXCL12.

Figure 6

Markers of systemic oxidative stress (A and B), C-reactive protein (CRP) (C), and chemokine CXCL12 (D). IGF-1 did not alter circulating monocyte subsets (E). Circulating N-tyrosine, CXCL12, and CRP levels were quantified by ELISA and TAC, by using colorimetric assay. TAC assay results shown in urinary acid (standard) equivalents (UAE). (E) Whole blood was mixed with a cocktail of antibodies against CD163-PE, CD14–Alexa Fluor 488, and porcine CD172a and subsequently with streptavidin-APC/Cy7. CD172a-positive leukocytes were size-gated and further differentiated into subsets based on CD163 and CD14 expression levels using FACS. n = 5 per time point per group for males and n = 9 for females for N-tyrosine, CRP, CXCL12, and monocyte assay. n = 5/males and females for TAC assay. *P < 0.05 and **P < 0.01 vs. saline, &P < 0.05 vs. males based on 3-way ANOVA.