Antibodies |
Topo IIbeta (H-8) |
Santa Cruz |
RRID: AB_628384 |
Topo IIbeta |
Abcam |
RRID: AB_2923318 |
Pan-Calcineurin A Antibody |
Cell Signaling Technology |
RRID: AB_2168458 |
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 |
MilliporeSigma |
RRID: AB_309864 |
Calcineurin A Polyclonal Antibody |
Thermo Fisher Scientific |
RRID: AB_10981308 |
Histone H2A.X antibody |
Abcam |
RRID: AB_297814 |
Goat Anti-Rabbit IgG Antibody, IRDye 800CW |
LI-COR |
RRID: AB_10796098 |
Lamin A/C (4C11) Mouse mAb (Alexa Fluor 488 Conjugate) |
Cell Signaling Technology |
RRID: AB_10997529 |
Phospho-Topoisomerase IIα (Ser1469) (D4F5) |
Cell Signaling Technology |
RRID: AB_2798109 |
Phospho-CK2 Substrate [(pS/pT) DXE] MultiMab™
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Cell Signaling Technology |
RRID: AB_2797653 |
Goat anti-Mouse IgG (H + L) Antibody, IRDye 680RD |
LI-COR |
RRID: AB_10956589 |
Actin beta |
Bio-Rad |
RRID: AB_2923317 |
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 |
Thermo Fisher Scientific |
RRID:AB_2534091 |
Goat anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor™ 594 |
Thermo Fisher Scientific |
RRID:AB_2534095 |
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 |
Thermo Fisher Scientific |
RRID:AB_2535805 |
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 |
Thermo Fisher Scientific |
RRID:AB_2535813 |
Lamin A/C |
Cell Signaling Technology |
RRID:AB_10545756 |
Anti-gamma H2A.X (phospho S139) antibody |
Abcam |
RRID:AB_303388 |
Bacterial and virus strains |
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Biological samples |
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Chemicals, peptides, and recombinant proteins |
Recombinant Human Calcineurin Protein |
R&D systems |
Cat#3160-CA |
Calmodulin (Human) (recombinant) |
Enzo |
Cat#BML-SE325–0001 |
Recombinant Human Topoisomerase II beta/TOP2B protein |
Abcam |
Cat#ab159736 |
KN-62 |
MilliporeSigma |
Cat#422706–1MG |
KT5720 |
MilliporeSigma |
Cat#420323–50UG |
Dantrolene, sodium salt |
Tocris |
Cat#0507 |
InSolution™ Okadaic Acid, Prorocentrum |
MilliporeSigma |
Cat#495609–25UG |
Nifedipine |
Tocris |
Cat#1075 |
2 aminoethoxydiphenyl borate (2APB) |
Tocris |
Cat#1224 |
MEK/ERK inhibitor |
Tocris |
Cat#PD98059 |
Calcineurin Inhibitor VIII, CN585 - |
MilliporeSigma |
Cat#207003–10MG |
Tacrolimus (FK506) |
Selleckchem |
Cat#S5003 |
D-AP5 |
Tocris |
Cat#0106 |
Critical commercial assays |
Nick Translation DNA Labeling System |
ENZO |
Cat#ENZ-GEN111–0050 |
Duolink® In Situ Detection Reagents FarRed |
MilliporeSigma |
Cat# DUO92013 |
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Deposited data |
Targeted Mass Spectrometry (PRM LC – MS/MS) |
This paper |
MassIVE: MSV000088392 |
Mass Spectrometry (LC – MS/MS of immunoprecipitated Top2B) |
This paper |
MassIVE: MSV000089825 |
Mendeley Dataset - Unprocessed blots, gels, and microscopy images |
This paper |
DOI: 10.17632/8697s83wpv.1
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Experimental models: Cell lines |
NIH 3T3 |
ATCC |
Cat#CVCL_0594 |
HEK293 |
ATCC |
Cat#CVCL_0045 |
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Experimental models: Organisms/strains |
Mouse C57BL/6J |
The Jackson Laboratory |
Cat#000664 |
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Oligonucleotides |
Fos Exon Forward: GCCAACTTTATCCCCACGGT |
This paper |
N/A |
Fos Exon Reverse: TCTTCACCATTCCCGCTCTG |
This paper |
N/A |
Npas4 Exon Forward: GAGCAAGAGCCTGAGCGAAAAG |
This paper |
N/A |
Npas4 Exon Reverse: CCAGCAAAGAAGACACCCTTG |
This paper |
N/A |
GAPDH Forward: CTCTCCTCCTCCCTGTTCC |
This paper |
N/A |
GAPDH Reverse: TCCCTAGACCCGTACAGTGC |
This paper |
N/A |
bglobin Forward: TGACCAATAGTCTCGGAGTCCTG |
This paper |
N/A |
bglobin Reverse: AGGCTGAAGGCCTGTCCTTT |
This paper |
N/A |
Fos Forward: GAACGGAATAAGATGGCTGC |
This paper |
N/A |
Fos Reverse: TTGATCTGTCTCCGCTTGG |
This paper |
N/A |
FosB Forward: CCCGAGAAGAGACACTTACC |
This paper |
N/A |
FosB Reverse: CTCTTCAAGCTGATCAGTTTCC |
This paper |
N/A |
Npas4 Forward: CTGCATCTACACTCGCAAGG |
This paper |
N/A |
Npas4 Reverse: GCCACAATGTCTTCAAGCTCT |
This paper |
N/A |
Egr1 Forward: AGCGAACAACCCTATGAGCA |
This paper |
N/A |
Egr1 Reverse: TCGTTTGGCTGGGATAACTC |
This paper |
N/A |
GAPDH Forward: CTCTCCTCCTCCCTGTTCC |
This paper |
N/A |
GAPDH Reverse: TCCCTAGACCCGTACAGTGC |
This paper |
N/A |
Nr4a1 Forward: ATGCCTCCCCTACCAATCTTC |
This paper |
N/A |
Nr4a1 Reverse: CACCAGTTCCTGGAACTTGGA |
This paper |
N/A |
ActB Forward: GGCTGTATTCCCCTCCATCG |
This paper |
N/A |
ActB Reverse: CCAGTTGGTAACAATGCCATGT |
This paper |
N/A |
HA-tagged mouse DNA topoisomerase IIβ |
Addgene |
Cat# pYLL201 |
Recombinant DNA |
Fos
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CHORI (Children’s Hospital Oakland Research Institute) |
RP24–208N11 |
Fos (upstream)
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CHORI (Children’s Hospital Oakland Research Institute) |
RP24–368B17 |
Fos (downstream)
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CHORI (Children’s Hospital Oakland Research Institute) |
RP24–178I11 |
FosB
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CHORI (Children’s Hospital Oakland Research Institute) |
RP24–161J12 |
ActB
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CHORI (Children’s Hospital Oakland Research Institute) |
RP24–266N |
Malat1
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CHORI (Children’s Hospital Oakland Research Institute) |
RP24–290K10 |
Npas4
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CHORI (Children’s Hospital Oakland Research Institute) |
RP24–238I5 |
Gm50442
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CHORI (Children’s Hospital Oakland Research Institute) |
RP24–344E18 |
Software and algorithms |
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Other |
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