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. Author manuscript; available in PMC: 2023 Oct 20.
Published in final edited form as: Mol Cell. 2022 Oct 6;82(20):3794–3809.e8. doi: 10.1016/j.molcel.2022.09.012

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Topo IIbeta (H-8) Santa Cruz RRID: AB_628384
Topo IIbeta Abcam RRID: AB_2923318
Pan-Calcineurin A Antibody Cell Signaling Technology RRID: AB_2168458
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 MilliporeSigma RRID: AB_309864
Calcineurin A Polyclonal Antibody Thermo Fisher Scientific RRID: AB_10981308
Histone H2A.X antibody Abcam RRID: AB_297814
Goat Anti-Rabbit IgG Antibody, IRDye 800CW LI-COR RRID: AB_10796098
Lamin A/C (4C11) Mouse mAb (Alexa Fluor 488 Conjugate) Cell Signaling Technology RRID: AB_10997529
Phospho-Topoisomerase IIα (Ser1469) (D4F5) Cell Signaling Technology RRID: AB_2798109
Phospho-CK2 Substrate [(pS/pT) DXE] MultiMab Cell Signaling Technology RRID: AB_2797653
Goat anti-Mouse IgG (H + L) Antibody, IRDye 680RD LI-COR RRID: AB_10956589
Actin beta Bio-Rad RRID: AB_2923317
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 Thermo Fisher Scientific RRID:AB_2534091
Goat anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 594 Thermo Fisher Scientific RRID:AB_2534095
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific RRID:AB_2535805
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific RRID:AB_2535813
Lamin A/C Cell Signaling Technology RRID:AB_10545756
Anti-gamma H2A.X (phospho S139) antibody Abcam RRID:AB_303388
Bacterial and virus strains
 
 
 
 
 
Biological samples
 
 
 
 
 
Chemicals, peptides, and recombinant proteins
Recombinant Human Calcineurin Protein R&D systems Cat#3160-CA
Calmodulin (Human) (recombinant) Enzo Cat#BML-SE325–0001
Recombinant Human Topoisomerase II beta/TOP2B protein Abcam Cat#ab159736
KN-62 MilliporeSigma Cat#422706–1MG
KT5720 MilliporeSigma Cat#420323–50UG
Dantrolene, sodium salt Tocris Cat#0507
InSolution Okadaic Acid, Prorocentrum MilliporeSigma Cat#495609–25UG
Nifedipine Tocris Cat#1075
2 aminoethoxydiphenyl borate (2APB) Tocris Cat#1224
MEK/ERK inhibitor Tocris Cat#PD98059
Calcineurin Inhibitor VIII, CN585 - MilliporeSigma Cat#207003–10MG
Tacrolimus (FK506) Selleckchem Cat#S5003
D-AP5 Tocris Cat#0106
Critical commercial assays
Nick Translation DNA Labeling System ENZO Cat#ENZ-GEN111–0050
Duolink® In Situ Detection Reagents FarRed MilliporeSigma Cat# DUO92013
 
 
 
 
Deposited data
Targeted Mass Spectrometry (PRM LC – MS/MS) This paper MassIVE: MSV000088392
Mass Spectrometry (LC – MS/MS of immunoprecipitated Top2B) This paper MassIVE: MSV000089825
Mendeley Dataset - Unprocessed blots, gels, and microscopy images This paper DOI: 10.17632/8697s83wpv.1
 
 
Experimental models: Cell lines
NIH 3T3 ATCC Cat#CVCL_0594
HEK293 ATCC Cat#CVCL_0045
 
 
 
Experimental models: Organisms/strains
Mouse C57BL/6J The Jackson Laboratory Cat#000664
 
 
 
 
 
Oligonucleotides
Fos Exon Forward: GCCAACTTTATCCCCACGGT This paper N/A
Fos Exon Reverse: TCTTCACCATTCCCGCTCTG This paper N/A
Npas4 Exon Forward: GAGCAAGAGCCTGAGCGAAAAG This paper N/A
Npas4 Exon Reverse: CCAGCAAAGAAGACACCCTTG This paper N/A
GAPDH Forward: CTCTCCTCCTCCCTGTTCC This paper N/A
GAPDH Reverse: TCCCTAGACCCGTACAGTGC This paper N/A
bglobin Forward: TGACCAATAGTCTCGGAGTCCTG This paper N/A
bglobin Reverse: AGGCTGAAGGCCTGTCCTTT This paper N/A
Fos Forward: GAACGGAATAAGATGGCTGC This paper N/A
Fos Reverse: TTGATCTGTCTCCGCTTGG This paper N/A
FosB Forward: CCCGAGAAGAGACACTTACC This paper N/A
FosB Reverse: CTCTTCAAGCTGATCAGTTTCC This paper N/A
Npas4 Forward: CTGCATCTACACTCGCAAGG This paper N/A
Npas4 Reverse: GCCACAATGTCTTCAAGCTCT This paper N/A
Egr1 Forward: AGCGAACAACCCTATGAGCA This paper N/A
Egr1 Reverse: TCGTTTGGCTGGGATAACTC This paper N/A
GAPDH Forward: CTCTCCTCCTCCCTGTTCC This paper N/A
GAPDH Reverse: TCCCTAGACCCGTACAGTGC This paper N/A
Nr4a1 Forward: ATGCCTCCCCTACCAATCTTC This paper N/A
Nr4a1 Reverse: CACCAGTTCCTGGAACTTGGA This paper N/A
ActB Forward: GGCTGTATTCCCCTCCATCG This paper N/A
ActB Reverse: CCAGTTGGTAACAATGCCATGT This paper N/A
HA-tagged mouse DNA topoisomerase IIβ Addgene Cat# pYLL201
Recombinant DNA
Fos CHORI (Children’s Hospital Oakland Research Institute) RP24–208N11
Fos (upstream) CHORI (Children’s Hospital Oakland Research Institute) RP24–368B17
Fos (downstream) CHORI (Children’s Hospital Oakland Research Institute) RP24–178I11
FosB CHORI (Children’s Hospital Oakland Research Institute) RP24–161J12
ActB CHORI (Children’s Hospital Oakland Research Institute) RP24–266N
Malat1 CHORI (Children’s Hospital Oakland Research Institute) RP24–290K10
Npas4 CHORI (Children’s Hospital Oakland Research Institute) RP24–238I5
Gm50442 CHORI (Children’s Hospital Oakland Research Institute) RP24–344E18
Software and algorithms
 
 
 
 
 
Other