FIG. 6.

Evidence that a suppressing mutation resides in an xpd allele. (A) Fifty micrograms of soluble protein from each extract was resolved by SDS-PAGE, and XPD protein was identified by Western blotting using an affinity-purified anti-XPD polyclonal antibody. In some lanes, the XPD protein appears as a doublet, for reasons not understood. (B) UV survival curves for cells of the WT (○), V-H1 (□), revertant RH1-53 (◊), V-H1 transfected with XPD^WT (●), V-H1 transfected with XPD^RH1-53 (⧫), and RH1-53 transfected with XPD^T46I (▪). Each survival curve represents the average of two experiments. Error bars represent SEMs.