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. 2023 Mar 7;12:e84632. doi: 10.7554/eLife.84632

Figure 8. Fragments bind at new sites only at room temperature (RT).

(A–C) First example. (A) The two sites are ~38 Å away from one another. (B) In the RT dataset (z0042), the RT event map, calculated with 1-BDC of 0.36 and contoured at 1.5 σ (red), supports a bound fragment in the RT model (red) at a new site while the cryogenic (cryo) model (y1525) (blue) has no bound fragment. (C) By contrast, the RT event map (same contour) does not show any density for the cryo model (blue) from the previous cryo dataset (y1525). (D–F) Second example. (D) The two sites are ~46 Å away from one another. (E) The RT event map contoured at 1.75 σ (red) (same contour) does not support the cryo model (blue) from the previous cryo dataset (y0572). (F) By contrast, at a new site the RT event map (same contour) supports a bound fragment in the RT model (x0225) (red). The cryo model has no bound fragment.

Figure 8.

Figure 8—figure supplement 1. Only half of a cryogenic (cryo) stacking artifact disappears at room temperature (RT).

Figure 8—figure supplement 1.

(A) Cryo y0660 (blue); cryo density (blue) 1.5 σ. (B) RT z0115 (red); RT density (red) 0.7 σ.
Figure 8—figure supplement 2. Fragment that binds at a new site only at room temperature (RT).

Figure 8—figure supplement 2.

(A) The two sites are ~40 Å away from one another. (B) In the RT event map contoured at 2.5 σ (red), the RT dataset (x0285) supports a bound fragment at a new site. The cryogenic (cryo) model (blue) from the previous cryo dataset (y0772) has no bound fragment. (C) By contrast, the RT event map, calculated with 1-BDC of 0.15 (same contour), does not support the cryo model. (The site where the RT map matches the cryo site is not shown.)