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Inhibition of E12-dependent p21 induction by Twist and Ids overexpression. MG63 cells were transfected with pCMV-E12 and either pCMV-Twist (A), pCMV-Id1 (B), or pCMV-Id2 (C). DNA amounts used for transfection were adjusted by empty vectors. The cells were maintained in DMEM containing 10% FBS for 72 h. The cells were fixed and stained for E12 (rhodamine; a), p21 (fluorescein; b), and DNA (DAPI; c). Arrowheads, cells expressing E12. Scale bar, 20 μm.
