TABLE 1.
Cell cycle distribution of serum-starved and 1,25-(OH)2D3-treated MG63 cellsa
| Time (h) | Percentage of cells in S and G2/M at indicated FBS concn (%) and 1,25-(OH)2D3 statusb
|
||||
|---|---|---|---|---|---|
| 10
|
2
|
0.1c | |||
| − | + | − | + | ||
| 24 | 32.9 | 32.4 | 26.1 | 22.7 | nd |
| 48 | 25.5 | 20.5 | 13.7 | 10.2 | 8.3 |
| 72 | 15.7 | 15.1 | 13.6 | 12.1 | 7.6 |
a
MG63 cells were cultured in DMEM with FBS at the concentrations indicated. The cells were treated with or without 1,25-(OH)2D3 for the indicated times and analyzed for DNA content by propidium iodide staining with a flow cytometer. Data were processed using Cell Quest. nd, not determined.
b
+, present; −, absent.
c
1,25-(OH)2D3 was absent at this FBS concentration.