TABLE 2.
Effects of E12 expression on DNA synthesis and p21 expressiona
| Transfection | % of cells that were:
|
|||
|---|---|---|---|---|
| E12+ and:
|
E12− and:
|
|||
| p21+ | BrdU− | p21+ | BrdU− | |
| E12 | 88.3 | 81.4 | 0.1 | 43.7 |
| E12 + p21as | 15.0 | 27.6 | 0.1 | 51.0 |
a
MG63 cells were transiently transfected with 5.0 μg of pCMV-E12 and either an empty vector or pCMV-p21 antisense (p21as). The cells were maintained in DMEM containing 10% FBS. The expression of p21 was monitored by immunostaining of E12 and p21 (Fig. 4A and C). DNA synthesis was evaluated by measuring the uptake of BrdU for 1 h in a parallel dish of cells, and cells were stained for E12 (Fig. 4B and D). At least 150 cells were counted in each category, and results are representative of multiple experiments performed.